Changes in peripheral blood and bone marrow erythroblasts in rats after hypoxic exposure

Xiong Hua; Li Jinjie; Wang Lu; Ma Jie

doi:10.12307/2025.526

Abstract：BACKGROUND:The changes in peripheral blood erythrocytes of rats exposed to hypoxia are related to changes in proliferation of erythroblasts in the bone marrow.Related regulatory factors such as hypoxia-inducible factor and erythropoietin are involved. OBJECTIVE:To explore the changes and related factors of peripheral blood erythrocytes and bone marrow erythroblasts in rats after hypoxic exposure. METHODS:An altitude of 5 000 meters was simulated to establish an experimental rat model under a low-pressure and low-oxygen environment.The control group was raised in a laboratory at an altitude of 2 260 meters.The blood routine,bone marrow erythroblast ratio,hypoxia-inducible factor,and erythropoietin of rats were compared before and after hypoxic exposure. RESULTS AND CONCLUSION:(1)The peripheral blood erythrocyte count,hemoglobin,hematocrit and other indicators of the experimental group rats were higher than those of the control group(P＜0.05).(2)The proportion of bone marrow CD71+erythroblasts of the experimental group rats increased compared with the control group(P＜0.05).(3)The expression level of hypoxia-inducible factor-2α in bone marrow CD71+erythroblasts of experimental group rats increased compared with the control group(P＜0.05).(4)The erythropoietin level in the bone marrow supernatant of the experimental group rats was higher than that of the control group(P＜0.05).The results indicate that the proportion of peripheral blood erythrocytes and bone marrow erythroblasts in rats increases after hypoxic exposure,which may be related to the increased levels of hypoxia-inducible factor-2α in bone marrow erythroblasts and erythropoietin in bone marrow supernatant.