DOI: 10.12307/2025.076
Comparison of biological characteristics of adipose-derived stem cells in young and old mice
Lin Meiyu
Yao Xiang
Gao Jing
Zhao Xilong
Pan Xinghua
Ruan Guangping
Abstract：BACKGROUND:Adipose-derived stem cells have anti-aging effects,but whether adipose-derived stem cells from donors of different ages are different needs further study.
OBJECTIVE:To compare the biological properties of adipose-derived stem cells in old and young mice.
METHODS:Adipose-derived stem cells were extracted from adipose tissue of C57BL mice aged 8 and 14 weeks,respectively.The differences of cell cycle,apoptosis,and proliferation of adipose-derived stem cells in old and young mice were compared.The expression levels of aging-related P21 and P27 genes and proteins of adipose-derived stem cells in old and young mice were detected by quantitative PCR and western blot assay.
RESULTS AND CONCLUSION:Compared with old mouse adipose-derived stem cells,young mouse adipose-derived stem cells were more active,more regular in morphology,less apoptosis,faster proliferation,and lower in expression of age-related P21 and P27 genes and proteins.It has been proven that adipose-derived stem cells from young mice have better anti-aging effects.
Keywords：adipose-derived stem celladipose tissueyoungold agemouseanti-aging
Publication Date：2025-07-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 4063-4068 )