DOI: 10.12307/2025.375
Metabolomics analysis of the lumbar spine after alendronate sodium intervention in ovariectomized rats with osteoporosis
Chen Xinfei
Dai Yahui
Xie Bingying
Huang Xiaobin
Huang Huimin
Huang Jingwen
Li Shengqiang
Ge Jirong
Abstract：BACKGROUND:Studies have reported that alendronate intake significantly increases bone mineral density in patients with osteoporosis.
OBJECTIVE:To analyze and compare the changes in metabolites before and after alendronate intervention in ovariectomized rats by chromatography-mass spectrometry,and to further explore the specific mechanism and target of alendronate in the treatment of osteoporosis.
METHODS:A total of 36 female Sprague-Dawley rats were randomly divided into model group,alendronate sodium group and sham operation group.The osteoporosis model was established by ovariectomy in the first two groups.Four weeks after modeling,the rats in the alendronate group were intragastrically given alendronate sodium,while those in the sham operation group and model group were given equal volume of normal saline.After 12 weeks of continuous gavage,the metabolites of the lumbar spine were analyzed by chromatography-mass spectrometry,and the common differential metabolites were obtained,which were analyzed by bioinformatics such as Kyoto Gene and Genome Encyclopedia pathway.
RESULTS AND CONCLUSION:Totally 17 different metabolites were obtained in the three groups.The enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes showed that alendronate sodium could regulate unsaturated fatty acid biosynthesis,linoleic acid metabolism and other pathways to protect ovariectomized rats.These results indicate that alendronate sodium may exert its anti-osteoporosis effect by interfering with unsaturated fatty acid bioanabolism and linoleic acid metabolism,so as to achieve the purpose of preventing osteoporosis
Keywords：osteoporosismetabolomicsalendronate sodiumlinoleic acid metabolismunsaturated fatty acid biosynthesisdifferential metabolitechromatography-mass spectrometrytraditional Chinese medicine
Publication Date：2025-04-11
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 2277-2284 )