Menadione-7 induces the differentiation of rat bone marrow stromal cells in vitro

Xu Bing; Liu Yuan

doi:10.3969/j.issn.2095-4344.0431

Abstract：BACKGROUND: The current research on menadione 7 mainly focuses on its anti-osteoporosis effect, and its role in rat bone marrow stromal cells in vitro culture system is unclear. OBJECTIVE: To investigate the effect of menadione-7 on the proliferation and differentiation of Bone marrow stromal cells, and to provide theoretical basis for the application of menadione-7 in bone tissue engineering. METHODS:Bone marrow stromal cells were obtained from rats by surgery.After cultured in vitro,bone marrow stromal cells were incubated in cell culture dishes. The proliferation of bone marrow stromal cells was induced by 10-6, 10-5and 10-4g/L menadione-7 and 100 μg/L basic fibroblast growth factor (bFGF). The corresponding absorbance value of bone marrow stromal cells was detected in each group. The levels of alkaline phosphatase and the number of calcium nodules in bone marrow stromal cells were detected at 2, 4, 6 and 8 days, and the mRNA expression of osteocalcin, osteopontin and Runx2 was detected at 4, 6 and 8 days. RESULTS AND CONCLUSION: Bone marrow stromal cells induced by menadione-7 proliferated well, and the cell proliferation could be promoted by different concentrations of menadione-7 and bFGF (P < 0.05). More alkaline phosphatase and calcium nodules were expressed in menadione-7 groups than the control group. The mRNA expression of osteocalcin, osteopontin and Runx2 could be significantly up-regulated by 10-4g/L menadione-7 (P < 0.05). To conclude, bone marrow stromal cells can be induced by menadione-7 into osteogenic differentiation and menadione-7 can be used as an inducer for seed cells in bone tissue engineering.