Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro
Zhang Jue
Xue Si-liang
Luo Yuan
Zhi Wei
Abstract：BACKGROUND: Tetracycline hydrochloride is shown to promote viability and proliferation of human embryonic osteoblasts cultured in vitro, increase the production of type I collage and osteocalcin. OBJECTIVE: To study the proliferation activity of bone marrow mesenchymal stem cells (BMSCs) cultured by tetracycline hydrochloride in vitro. METHODS: Passage 3 BMSCs from Sprague-Dawley rats were cultured and divided into two groups: cells in control group were cultured in complete medium containing fetal bovine serum, and those in experimental group were cultured in complete medium containing tetracycline hydrochloride. After days 1-8 of culture, MTT and cell counting assay were used to detect cell viability and proliferation in the two groups. RESULTS AND CONCLUSION: (1) Cell counting assay: with time, the number of cells in both groups was increased, but the cell number in the experimental group was higher than that in the control group at days 4-8 of culture (P < 0.05). (2) MTT assay: With time, the number of cells in both groups was increased: incubation period was at day 1, logarithmic growth period was within days 3-6, and plateau period began at day 7. Then, the cells continued to proliferate, and the cell proliferation was higher in the experimental group than the control group at days 5-8 of culture (P < 0.05). To conclude, tetracycline hydrochloride can significantly enhance BMSCs proliferation.
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Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5249-5254 )