Biocompatibility of titanium dioxide nanotube bio-dialysis membrane

Gao Yu-han; Wang Sheng-nan; Wang Biao-baio

doi:10.3969/j.issn.2095-4344.2017.26.012

Abstract：BACKGROUND: Traditional dialysis membranes are mostly synthesized by organic polymer materials. Although these materials can meet the needs of treatment, the pore size range is large and the microtubules are not uniform, which can cause protein clogging on the dialysis membrane. Therefore, it is difficult to achieve the expected therapeutic effect.OBJECTIVE: To study the hemocompatibility of titanium dioxide nanotube bio-dialysis membrane.METHODS: High-strength titanium dioxide nanotube films were prepared by anodic oxidation method, the bottom of the nanotubes was etched by HF gas, and the LLC-PK1 and ECV304 cells were seeded onto the prepared titanium dioxide nanotube array membranes to construct two kinds of titanium dioxide nanotube bio-dialysis membranes. Hemolysis test,dynamic clotting time test and platelet adhesion test were used to determine the hemocompatibility of the prepared titanium dioxide nanotube bio-dialysis membrane.RESULTS AND CONCLUSION: In the hemolysis test, the hemolysis rates of the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells were lower than that of the polyurethane material (0.30％, 0.34％,0.56％; P < 0.05). In the dynamic clotting test (20, 40, 70 minutes), the dynamic clotting time of the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells were significantly longer compared with the polyurethane material (P < 0.05). In the platelet adhesion test, the platelet adhesion rate showed no significant difference between the polyurethane material and the titanium dioxide nanotube array membranes carrying LLC-PK1 and ECV304 cells (P > 0.05). These findings indicate that the titanium dioxide nanotube bio-dialysis membrane possesses good hemocompatibility.