A novel tissue-engineered crosslinked collagen-chitosan scaffold and its biocompatibility with nerve cells
Abstract：BACKGROUND:Treatment for peripheral nerve injury is still a chalenge in the field of regenerative medicine. The use of scaffold materials is considered to be one of the promising opportunities to settle these defects. The ideal scaffold material should be safe and durable, but have no antigenicity.OBJECTIVE: To develop a novel tissue-engineered colagen-chitosan composite scaffolds under modification in attempt to repair sciatic nerve injury in rats.METHODS:First the collagen-chitosan composite scaffold was prepared, and then cross-linked with 1-ethyl-3-(3-2 methyl amino propyl)-WC 2 imide (EDC) and N-hydroxy succinimide (NHS). After modification, nerve cells were seeded onto the scaffold. Finally, the cell-scaffold composite was implanted into the injured sciatic nerve.RESULTS AND CONCLUSION:The EDC/NHS-crosslinked collagen-chitosan scaffold showed a three-dimensional reticular structure. Four weeks after the cell-scaffold composite implantation, neurofilament protein 200 was found to express in the injured sciatic nerve. This indicates that the crosslinked colagen-chitosan scaffold has good cytocompatibility.
Keywords：Biocompatible MaterialsCollagenChitosanTissue Engineering
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 2913-2919 )