Effect of continuous versus intermittent force on the proliferation and differentiation of bone marrow stromal stem cells

doi:10.3969/j.issn.2095-4344.2017.13.001

Abstract：BACKGROUND:In the process of distraction osteogenisis, continuous and intermittent force can be used. However,which one is more beneficial for bone formation is still controversial.OBJECTIVE:To investigate and compare the effect of continuous and intermittent force on the proliferation and differentiation of bone marrow stromal stem cells (BMSCs).METHODS:Rabbit BMSCs were obtained using density gradient centrifugation method. The passage 3 BMSCs were seeded onto 6-well cell culture plates for mechanical investigation. Then, the cells were randomly divided into three groups. Group A was a control group without any treatments. Group B was the continuous force group with a centrifugal force (1100 r/min, relative centrifugal force 20×g) applied 4 hours per day. Group C was the intermittent group with an centrifugal force (1100 r/min, relative centrifugal force 100×g) applied twice in 4 hours and lasting 24 minutes once. After 3 days of culture under mechanical force, the proliferation of BMSCs and the expression of alkaline phosphatase and osteocalcin were detected and compared.RESULTS AND CONCLUSION:The centrifugal force promoted the ostegenic differentiation and proliferation of BMSCs and the expression of alkaline phosphatase significantly increased compared with the control group (P < 0.05).Compared with the intermittent force, the continuous force showed better effect on osteogenic differentiation and proliferation of BMSCs as well as the activity of alkaline phosphatase (P < 0.05).