Therapeutic effect of bone marrow mesenchymal stem cells on multiple organ dysfunction syndrome

doi:10.3969/j.issn.2095-4344.2017.09.013

Abstract：BACKGROUND: Clinical treatment of multiple organ dysfunction syndrome has been a big problem today, whilethe treatment of bone marrow mesenchymal stem cells may provide a new research direction.OBJECTIVE: To investigate the therapeutic effects of bone marrow mesenchymal stem cells transplanted intomultiple organ dysfunction syndrome models.METHODS: According to body weight of rats, endotoxin in the 1 mg/kg dose was injected into the femoral vein tobuild the multiple organ dysfunction syndrome models in rats, and the surviving rats were randomly divided intotreatment and control groups. Then the rats in the treatment group were injected intravenously third-generationbone marrow mesenchymal stem cells (5×106), while rats in the control group injected intravenously with an equalvolume of saline. After 5 days of treatment, the blood routine examination, blood biochemistry and coagulationtests were conducted, and then the liver, kidney, heart, and spleen of rats were captured to observed pathologicalchanges through the hematoxylin-eosin staining.RESULTS AND CONCLUSION: One week after cell transplantation, liver function and biochemical indicatorswere improved significantly in the treatment group compared with the control group (P < 0.05), and the whiteblood cell count in the treatment group was closer to normal levels (P < 0.05). Hematoxylin-eosin staining resultsshowed that in the multiple organ dysfunction syndrome rats appeared to have different degrees of inflammatorylesions and necrosis in the heart, liver, spleen, and kidneys, while inflammatory infiltration and necrotic lesionsdecreased significantly in the treatment group compared with the control group. To conclude, bone marrowmesenchymal stem cells exert remarkable therapeutic effects on multiple organ dysfunction syndrome.