Enzyme digestion method versus tissue block method in the culture of cardiac stem cells
Abstract：BACKGROUND:At present, various methods of extracting cardiac stem cel s have been reported in China, but the processes of isolation, culture and purification are not yet standardized. OBJECTIVE:To explore the difference in the culture of rat cardiac stem cel s using enzyme digestion method and tissue block method. METHODS:Cardiac stem cel s from Sprague-Dawley rats, clean grade, 1 day of age, were extracted and cultured in vitro using the enzyme digestion method (control group) or tissue block method (experimental group). RESULTS AND CONCLUSION:Both of the two methods could produce cardiac stem cel s, but the proportion of cultured cel s in the experimental group was significantly higher than that in the control group, indicating the tissue block method is superior to the enzyme digestion method in the culture of cardiac stem cel s.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 7565-7570 )