Cloning of rat C-sis gene and construction of its eukaryotic expression vector
Sun Guo-fang
Ding Hao
Abstract：BACKGROUND:C-sis proto-oncogene can promote tissue repair by inducing cel proliferation and inhibiting cel apoptosis. Therefore, C-sis may play a positive role in the repair of damaged liver tissue and the treatment of fulminant hepatic failure. <br> OBJECTIVE:To construct pcDNA3.1/C-sis eukaryotic expression vector and detect its expression in BRL cel s (the normal liver cel s of rats) and rat liver cel s in vivo. <br> METHODS:The ful-length coding sequence of C-sis gene was cloned through real time-PCR. pcDNA3.1/C-sis eukaryotic expression vector was constructed and sequenced, fol owed by transfected into BRL cel s using liposome and injected into the rat liver via tail vein. Final y, its expression in BRL cel s and rat liver cel s in vivo was identified by fluorescence quantitative PCR and western blotting. <br> RESULTS AND CONCLUSION:(1) The ful length of encoding region of C-sis gene was successful y cloned. Sequencing proved that pcDNA3. 1/C-sis recombinant eukaryotic expression vector was constructed successful y. (2) The expression of C-sis was increased after transfected into BRL cel s and rat liver. (3) These results provide basis for the subsequent study of the effect of C-sis gene on fulminant hepatic failure in rats.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 7418-7424 )