Protective effects of ginsenoside Rb3 on a rat model of ischemia/reperfusion injury
Wu Hui-zhen
Jia Qing-zhong
Abstract：BACKGROUND:Ginsenoside Rb3 has a variety of physiological activities, which mainly reflected in the cardiovascular treatment. <br> OBJECTIVE:To study the protective effects of ginsenoside Rb3 on the ischemia/reperfusion injury of rats. <br> METHODS:120 Sprague-Dawley rats were randomly assigned to six groups, with 20 in each group. Rats in the model and sham operation groups were intragastrical y given physiological saline 2 mL/kg?d for 2 consecutive days. Rats in the positive drug group were intragastrical y given diltiazem 2 mL/kg?d for 2 consecutive days. Rats in the low-dose drug group, moderate-dose drug group and high-dose drug group were intragastrical y given ginsenoside Rb3 10, 20, 30 mg/kg?d, for 2 consecutive days. At 2 days after administration, the chest of rats in the sham operation was opened, but these rats did not receive any other treatment. In other five groups, anterior descending branch of left coronary artery was ligated to establish models of ischemia/reperfusion injury. 48 hours later, we observed pathological sections of rat cardiac muscle, calculated percentage of organ coefficient and myocardial infarction area, measured the levels of serum isozyme, malondialdehyde, lactate dehydrogenase, endothelial relaxing factor, superoxide dismutase and glutathione peroxidase. <br> RESULTS AND CONCLUSION:(1) Pathological sections:ginsenoside Rb3 significantly improved the ischemia/reperfusion injury. (2) Percentages of organ coefficient and myocardial infarction area:compared with the model group, the percentages were significantly lower in the moderate-dose and high-dose drug groups (P<0.05, P<0.01). (3) Serum indexes:compared with the model group, ginsenoside Rb3 decreased isozyme, malondialdehyde, lactate dehydrogenase and endothelial relaxing factor levels in a dose-dependent manner, but increased superoxide dismutase and glutathione peroxidase levels in a dose-dependent manner. (4) Results suggested that ginsenoside Rb3 has protective effects on ischemia/reperfusion injury. Its mechanism of action may be associated with anti-lipid peroxide activities in cel s, the anti-free radical effects, anti-inflammatory functions and the influence of cardiac enzymes on energy metabolism.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 7320-7326 )