Glycolic acid loaded with chondrocytes repairs laryngeal cartilage defects

Jia Min; Yuan Kun

doi:10.3969/j.issn.2095-4344.2016.47.008

Abstract：BACKGROUND:A three-dimensional biodegradable scaffold is important for tissue-engineered cartilage construction, and it that can provide conditions for cel attachment and proliferation. OBJECTIVE:To observe the treatment outcomes of glycolic acid loaded with chondrocytes in laryngeal cartilage repair. METHODS:Sixty New Zealand white rabbits were enrol ed and randomly divided into control and <br> experimental groups. Laryngeal cartilage defect models were established in each group, fol owed by implanted with glycolic acid loaded with chondrocytes and glycolic acid, respectively. Gross and histological observations were conducted at 4 and 8 weeks after implantation. RESULTS AND CONCLUSION:Gross observation showed that at 4 weeks after implantation, a deep red wound with an obvious boundary was seen in the control group;the dark red and smooth defect paral el to the surrounding tissue was found in the experimental group. Toluidine blue staining revealed that at 8 weeks after implantation, the laryngeal defect site showed no obvious inflammation and cartilage col apse, with numerous newly-formed chondrocytes in the experimental group;in contrast, mild inflammation and cartilage col apse were found in the defect region of the control group, and few newly-formed chondrocytes appeared. The positive areas of glycosaminoglycan and type II col agen in the experimental group were significantly larger than those in the control group at 4 and 8 weeks after implantation (P<0.05). These results indicate that glycolic acid loaded with chondrocytes contributes to the repair of laryngeal cartilage defects.