Osteoinductive calcium phosphate ceramics repair alveolar cleft defects

Chen Hao-dong; Yao Jin-feng; Liang Zhi-gang

doi:10.3969/j.issn.2095-4344.2016.47.006

Abstract：BACKGROUND:In our previous studies, we had prepared calcium phosphate ceramics with better ectopic osteogenesis. OBJECTIVE:To explore the effect of novel osteoinductive calcium phosphate ceramics in the repairing of alveolar cleft. METHODS:Bilaterl alveolar defects were created in nine immature beagles. Three months later, osteoinductive calcium phosphate ceramics with high modular surface (experimental group) and smooth surface (control group) were randomly implanted in each side of the defect. Meanwhile, the corresponding material was implanted into the thigh muscle. New bone formation in the implanted region, osteogenesis in the implanted region and muscle, and respair results were respectively observed by fluorescence microscope, light microscope and CT at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION:(1) Fluorescence microscope observation:A circular permutation of red, yel ow and green fluorescent strip could be observed in both two groups. (2) Light microscope observation:At 12 weeks after implantation, in the experimental group, the bone reconstruction was obvious, the implant material was decomposed gradual y, the gap was fil ed with a large number of mature bone that combined with the rest material closely, and numerous Haversian canals appeared;the control group was similar but slightly inferior to the experimental group in the quality of new bone. The experimental group material successful y induced heterotopic osteogenesis in muscle, while the control did not. (3) CT examination:The two group materials restored the appearance and continuity of the alveolar ridge, and made no effect on the eruption of permanent teeth in both sides of the defect. To conclude, our findings suggest that the novel osteoinductive calcium phosphate ceramic exhibits advantages in alveolar cleft repair with earlier osteogenesis activation, faster osteogenesis rate and more bone formation than those traditional materials.