Effects of Bergapten on the aging of human umbilical vein endothelial cells induced by hydrogen peroxide
Ding Yan
Fu You
Shan Lan-lan
Nai Wen-qing
Wu Hong-yuan
Tang Liang
Chen Shun-zhi
Dai Meng
Abstract：BACKGROUND:The effective treatment of traditional Chinese medicine can al eviate the aging of endothelial cel s. Traditional Chinese medicine is difficult to extract, and in contrast, Bergapten is cheap and easily purified, but its antisenescence is little reported. OBJECTIVE:To explore the effect of Bergapten on H2O2-induced senescence of human umbilical vein endothelial cel s. METHODS:Human umbilical vein cel aging model was induced by H2O2, and pretreated with 0.1, 1 and 10μmol/L Bergapten, respectively. Expressions of pRb, pAmpk, pS6, LC3-II, and Beclin-1 protein in each group were detected by western blot assay, and the number of aging cel s and pS6-positive cel s was determined byβ-galactosidase staining and immunofluorescence, respectively. ESULTS AND CONCLUSION:After Bergapten pretreatment, pS6 and pRb protein expressions were significantly downregulated, and the number of cel s positive forβ-galactosidase and pS6 was significantly decreased, while protein expressions of pAmpk, Beclin-1and LC3-II were significantly increased (P<0.05). These findings suggest that Bergapten is able to delay the H2O2-induced aging of human umbilical vein endothelial cel s.
Machine-generated Keywords：
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 6885-6892 )