Platelet-rich plasma promotes bone mineralization during distraction osteogenesis

Shi Bo-wen; Li Da-wei; Gong Xian-bin; Ji Guo-qi; Feng Yuan-chao; Cai Cheng-kuo; Shu Heng-sheng

doi:10.3969/j.issn.2095-4344.2016.46.005

Abstract：BACKGROUND:The platelet-rich plasma contains various growth factors that could enhance the regeneration of certain tissues. OBJECTIVE:To observe the effect of platelet-rich plasma on the mineralization process during distraction osteogenesis in rabbits. METHODS:Thirty-two adult Japanese white rabbits were randomly divided into four groups (n=8 per group). The upper 1/3 tibial osteotomy at the posterior limb was performed in al rabbits, and the 1 cm limb lengthening models were prepared, fol owed by the local injection of 500μL platelet-rich plasma at 5, 15 and 25 days. The controls received no treatment. At 37 days after modeling, the rabbits were kil ed and newly formed cal us was removed to undergo hematoxylin-eosin staining, and the frontal X-ray film of tibia was obtained. RESULTS AND CONCLUSION:Lane-Sandhu radiographic scoring showed that the scores in the injection groups at 15 and 25 days after modeling were obviously better than those in the control group and the injection at 5 days after modeling. Lane-Sandhu histological scoring revealed that the scores in the injected groups at 15 and 25 days after modeling were significantly higher than those in the control group and group at 5 days after modeling (P<0.05), which highest at 25 days. These results suggest that local injection of platelet-rich plasma can promote bone mineralization during distraction osteogenesis effectively, especial y in the middle period of distraction osteogenesis.