Effect of Longbie Capsule on p38MAPK pathway and nuclear factor-kappa B p65 in chondrocytes of osteoarthritis
Pan Jian-ke
Cao Xue-Wei
Liu Jun
Xie Hui
Yang Wei-yi
Zhang Xian
Abstract：BACKGROUND:Previous studies found that Longbie Capsule can relieve knee joint swel ing of rats with knee osteoarthritis, and decrease the serum levels of interleukin-1β, interleukin-6 and interleukin-10. OBJECTIVE:To observe the influence of Longbie Capsule on p38MAPK pathway and nuclear factor-κB p65 (NF-κB p65) in chondrocytes of osteoarthritis. METHODS:Sixty Sprague-Dawley rats were equivalently randomized into five groups, and the rat models of right knee osteoarthritis were established in model, low-dose, middle-dose and high-dose groups. Three days later, rats in the latter three groups were respectively treated with 0.312 5, 0.625, and 0.937 5 g/kg Longbie Capsule via gastric lavage, twice daily for 4 consecutive weeks;while those in the control group received no intervention. At 2 and 4 weeks after treatment, the rat right knee joint was removed and made into paraffin tissue sections, and the expressions of MEK-3/6, p38, activating transcription factor 2 (ATF2), NF-κBp65, and their phosphorylated products of P-MEK-3/6, P-p38, P-ATF2, P-NF-κB p65 in cartilage tissue were detected by immunohistochemistry streptavidin-perosidase method. RESULTS AND CONCLUSION:After 2 and 4 weeks of treatment, chondrocytes in each group could express MEK-3/6, p38, ATF2, NF-κB p65, P-MEK-3/6, P-p38, P-ATF2 and P-NF-κB p65, and the percentage of positive cel s in the model group was significantly higher than that in the control group (P<0.01), and the treatment groups (P<0.05). These results indicate that Longbie Capsule cannot only inhibit the over expressions of MEK-3/6, p38, ATF2, NF-κB p65 and their phosphorylated products, but also inhibit the activation of p38MAPKs and NF-κB signaling pathway in chondrocytes of osteoarthritis, which is beneficial for cartilage degeneration and protecting the cartilage.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 6868-6877 )