Interventional effect of umbilical cord blood stem cell transplantation in rats with gestational hypertension

Cui Xue-mei; Jing Xiao-xiao

doi:10.3969/j.issn.2095-4344.2016.45.016

Abstract：BACKGROUND:With the depth understanding of mesenchymal stem cel s, mesenchymal stem cel s are found to exert a prominent effect on immune regulation and anti-inflammation. <br> OBJECTIVE:To investigate the therapeutic effect of umbilical cord blood stem cel transplantation on endotoxin-induced hypertension in pregnant rats. <br> METHODS:Twenty-four pregnant Sprague-Dawley rats were randomized into three groups with eight rats in each group:control, model and experimental groups. Endotoxin-induced hypertension models were made in the model and experimental groups. Meanwhile, rats were given intravenous injection of umbilical cord blood stem cel suspension (1 mL) in the experimental groups and the same volume of normal saline in the control and model groups. Therapeutic effects of umbilical cord blood stem cel transplantation were observed through detection of systolic blood pressure, urine protein level, serum white blood cel quantity and Ang II and ET-1 expression. <br> RESULTS AND CONCLUSION:Compared with the control group, the systolic blood pressure, urine protein level and serum white blood cel quantity of rats were increased significantly in the model group, and over time, endotoxin continuously promoted these parameters in the model group. After cel transplantation, a significant reduction in systolic blood pressure, urine protein level and serum white blood cel quantity of rats was found in the experimental group compared with the model group (P<0.05). After modeling, the expression levels of Ang II and ET-1 were decreased significantly, while these levels were increased significantly after cel transplantation (P<0.05). These findings indicate that umbilical cord blood stem cel transplantation may have a certain therapeutic effect on gestational hypertension in rats, which may be realized by regulating the secretion of endothelial injury-related factors.