Human amniotic mesenchymal stem cell transplantation for the repair of liver ischemia-reperfusion injury

Wang Xin-dang; Zhang Jian-jun; Ye Kui

doi:10.3969/j.issn.2095-4344.2016.45.015

Abstract：BACKGROUND:Studies have shown that human amniotic mesenchymal stem cel s can differentiate into hepatocyte-like cel s, suggesting that human amniotic mesenchymal stem cel transplantation provides a new potential for the clinical treatment of liver diseases. <br> OBJECTIVE:To observe the effect of human amniotic mesenchymal stem cel transplantation on the repair of liver ischemia-reperfusion injury repair. <br> METHODS:Sixty Sprague-Dawley rats were randomized into stem cel transplantation, model and control groups. Animal models of liver ischemia-reperfusion injury were made in the rats in the stem cel transplantation and model groups. One hour after modeling, rats in the stem cel transplantation were given injection of human amniotic mesenchymal stem cel s (0.5 mL, 106 cel s) via the tail vein, while rats in the model and control group were given L-DMEM (0.5 mL) or normal saline (0.5 mL), respectively. Liver function and liver morphology were detected at 1, 2, 3 weeks after transplantation. Meanwhile, RT-PCR detection and western blot assay were also conducted. <br> RESULTS AND CONCLUSION:(1) Liver function:Compared with the control group, levels of aspartate aminotransferase, alanine aminotransferase and malondialdehyde were significantly increased in the model group at different time points after transplantation (P<0.05), while a significant reduction in the levels of these three indicators was found after cel transplantation as compared with the model group (P<0.05). (2) Liver morphology:2 weeks after transplantation, rats in the model group exhibited hepatocyte degeneration and necrosis, and severe fibrosis, but these changes were remarkably al eviated in the stem cel transplantation group. (3) PT-PCR and western blot detection:2 weeks after transplantation, a significantly higher level of hepatocyte growth factor in the liver tissue and a lower level ofα-smooth muscle protein were found in the stem cel transplantation group compared with the model group (P<0.05). Al these experimental findings indicate that human amniotic mesenchymal stem cel transplantation can improve impaired liver function in rats, possibly through regulating hepatocyte growth factor andα-smooth muscle protein expression levels in the liver, and thereby promotes the repair of liver ischemia-reperfusion injury.