Optimal concentration of bone morphogenetic protein 2 used to inhibit adipogenic differentiation of bone marrow mesenchymal stem cells
Chen Ming
Zhang Tao
Liu Jun
Li Zhi-yun
Min Yan
Abstract：BACKGROUND:Bone morphogenetic protein 2 cannot only promote osteogenesis, but also at different concentration ranges regulate the differentiation of bone marrow mesenchymal stem cel s into adipocytes. Therefore, it is very important for the prevention and treatment of osteoporosis, if the range of concentrations of bone morphogenetic protein 2 can be found to regulate the differentiation of bone marrow mesenchymal stem cel s into adipocytes, after the induction of bone marrow mesenchymal stem cel s. <br> OBJECTIVE:To determine the optimal concentration of bone morphogenetic protein 2 to inhibit the differentiation of bone marrow mesenchymal stem cel s into adipocytes. <br> METHODS:Bone marrow samples were obtained from the femur and tibia of rats. Primary cultured bone marrow mesenchymal stem cel s were obtained by adherent culture method in vitro. Flow cytometry was used to identify the surface antigens of passage 2 bone marrow mesenchymal stem cel s. Cel s at passages 2 and 3 were taken and cultured in adipogenic induction medium for 3 days fol owed by the addition of 0.5, 5, 50, 100, 200μg/L bone morphogenetic protein 2, or culture medium of bone marrow mesenchymal stem cel s (control group), respectively. <br> RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s cultured were identified by the flow cytometry. Oil red O staining results showed that the increasing number of lipid droplets was shown in bone marrow mesenchymal stem cel s induced by bone morphogenetic protein 2 under the increasing concentration of 0.5 to 50μg/L;while the number of lipid droplets in bone marrow mesenchymal stem cel s decreased after 17 days of induction with bone morphogenetic protein 2 under the concentration of 100 to 200μg/L. These findings indicate that 100μg/L bone morphogenetic protein 2 can inhibit adipogenic differentiation of bone marrow mesenchymal stem cel , so as to reduce the formation of adipocytes in rats. Therefore, 100μg/L bone morphogenetic protein 2 is better to inhibit adipogenic differentiation of bone marrow mesenchymal stem cel s.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6720-6725 )