Promotion of bone fracture healing by bone marrow mesenchymal stem cells

Guo Qi-fa; Li Guang; Ren Rong; Li Ling-wei

doi:10.3969/j.issn.2095-4344.2016.45.002

Abstract：BACKGROUND:In recent years, the development of stem cel culture and isolation technologies provides new therapeutic choices for fracture healing. <br> OBJECTIVE:To investigate the effect of exogenous bone marrow mesenchymal stem cel s on bone fracture healing in traumatic fracture rats and on the migration ability of endogenous bone marrow mesenchymal stem cel s. <br> METHODS:Femoral fracture models were made in 48 Wistar rats and then randomized into experimental group and control group (n=24/group). Bone marrow mesenchymal stem cel s from another healthy rats were isolated using adherent method and then injected into the rats via the tail vein in the experimental group. Rats in the control group were given the same volume of normal saline. At 2, 3, 4, 8, 12 weeks after injection, we extracted bone marrow mesenchymal stem cel s from the femur of rats in the two groups. RT-qPCR was used to detect expression levels of type I col agen and CD44. Transwel method was used to detect cel migration ability. Immunohistochemitry method was employed to detect expression of nerve growth factors in the cal us. <br> RESULTS AND CONCLUSION:mRNA levels of type I col agen and CD44 in rat bone marrow mesenchymal stem cel s were significantly higher in the experimental group than the control group at 2, 3 and 4 weeks after injection (P<0.05). Compared with the control group, the higher migration ability of bone marrow mesenchymal stem cel s was found in the experimental group at 2 and 3 weeks after injection (P<0.05) as wel as the higher expression of nerve growth factor in the cal us in the experimental group at 3, 4, 8, 12 weeks after injection. Al these findings suggest that exogenous bone marrow mesenchymal stem cel s can improve the migration ability of endogenous bone marrow mesenchymal stem cel s and the expression of nerve growth factor in the cal us in rats with femoral fracture, thereby promoting fracture healing in rats.