Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro
Lu Wei
Guo Rui
Zhao Gang
Abstract：BACKGROUND:It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation. <br> OBJECTIVE:To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I col agen expression. <br> METHODS:Mouse osteoblast cel line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cel counting kit-8 assay, and alkaline phosphatase activity and type I col agen level measured using ELISA method. <br> RESULTS AND CONCLUSION:Different concentrations of Ligustrazine al significantly promoted the osteoblast proliferation and type I col agen level compared with the blank control group (P<0.005). The activity of alkaline phosphatase in al Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P<0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I col agen expression, with the optimum concentration of 10 mg/L.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5483-5488 )