Platelet-fibrin scaffoldfor treatment ofmyocardial infarction
Zhang Yan
Chen Yan
Li Qing-min
Meng Hua
Abstract：BACKGROUND:The development of biomaterials provides a new idea for myocardial infarction treatment, and so farthere are numerous biomaterials, such asfibrin glue, matrigel, emulsion of extracelular matrix and alginate,for myocardial infarction. But there is a lack of studiesabout the platelet-fibrin scaffold for myocardial infarction.
<br> OBJECTIVE:To explore the effect oftheplatelet-fibrin scaffold on rat myocardial infarction.
<br> METHODS:Platelet-fibrin was usedto prepare aplatelet-fibrin scaffold, and 40 rat models of myocardial infarction were randomly divided into two groups:200 μL platelet fibrins were injected into the tissues around the rat infarcted myocardium as platelet-fibrin scaffold group, and the same amount of DMEM was injected into the tissues around the rat infarcted myocardium as control group. Subsequently, expressions of vascular endothelial growth factor, hepatocyte growth factor and insulin-like growth factor 1released fromthe scaffold were detected.Production ofendothelial cels and cardiomyocytes, cardiomyocyte apoptosis,capilarydensity and cardiac functionin ratsafter implantationwere observed.
<br> RESULTS AND CONCLUSION:The platelet-fibrin scaffold was successfuly prepared. The expression of vascular endothelial growth factor, hepatocyte growth factor and insulin-like growth factor 1 in the scaffold groupwas (115.7±19.45), (23.98±12.32) and (288.4±16.74) ng/L, respectively. The number of von Wilebrand factor-positive cels and a-SA-positive cels in the platelet-fibrin scaffold groupwassignificantly higher thanthat inthe control group (P< 0.05). The number of TUNEL-positive cels in the platelet-fibrin scaffold group wassignificantly lower than thatinthe control group (P< 0.05). The capilary density and capilaries with blood flow in the platelet-fibrin scaffold group were significantly higher than thoseinthe control group (P< 0.05). Both the left ventricular end-diastolic volume and left ventricular end-systolic volume in the platelet-fibrin scaffold group were significantly lower than thoseinthe control group (P<0.05). In conclusion, the platelet-fibrin scaffold can promote the generationof endothelial cels, cardiomyocytes and capilaries, andfurtherimprove heart function. Besides, the platelet-fibrin scaffold exerts protective effect on the heart probably by secreting cytokines.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 5129-5135 )