Growth of human periodontal ligament cells on the poly-L-lactic acid hydroxyapatite
Gao Yong-zhi
Yin Zhi-yuan
Abstract：BACKGROUND:Inconstructing tissue-engineered periodontiumin vitto, the effective combination of seed cels with scaffold materials is the key to promote the quality of tissue-engineered periodontium,in which human periodontal ligament cels are commonly used.
<br> OBJECTIVE:To explore the growth of human periodontal ligament cels on the poly-L-lactic acid hydroxyapatite.
<br> METHODS:Human periodontal ligament cels were isolated and culturedin vitro, and passage 3 cels were chosen to be randomly divided into two groups: celscultured alone as control group, and cultured with poly-L-lactic acid hydroxyapatite as experimental group.After1, 2 and 3 days, alkaline phosphatase activity and expression of type III colagen in the two groups were detected, and the cel growth curve was depicted using MTT method.
<br> RESULTS AND CONCLUSION:By immunohistochemical staining,culturedcels were positive for vimentin and alkaline phosphatase staining and negative for anti-keratin staining, indicating that the cels wereidentifiedas human periodontal ligament cels. By MTT method, there was no significant difference in the absorbance value of two groups at different time points (P> 0.05). And after 1, 2 and 3-day co-culture, the alkaline phosphatase activity levels showed no significant differencebetween two groups(P> 0.05). Besides, no significant difference in the absorbance value of type III colagen was found in the two groups (P> 0.05). To conclude, the human periodontal ligament cels can grow and proliferate wel on the poly-L-lactic acid hydroxyapatite scaffold with no cytotoxicity.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 5082-5088 )