Copper ion concentration and angiogenesisas two correlates to abnomal uterine bleeding caused by copper/low-density polyethylene nanocomposite intrauterine device
Deng Ting
Li Qing
Chen Dan
Xie Xi
Abstract：BACKGROUND:Toachieve excelent contraceptive effects,the copper wire on the copper-containing intrauterine device surface persistently releases copper ions, but ectopic intrauterine device, abnormal uterine bleeding, menostaxis and waist and abdominal painareusualyinevitable.
<br> OBJECTIVE:To investigate the correlation of abnormal uterine bleedingcausedby copper/low-density polyethylenenanocompositeintrauterine devicewith copper ion concentration and expression of vascular endothelial growthfactor in endometrial tissues.
<br> METHODS:Totaly 60femalepatients, aged from 25 to 40 years old, hospitalizedfor taking intrauterine devices were enroled, and those patients were divided into abnormal uterine bleeding group (n=32) and non-bleeding group (n=28). In the meanwhile,another20women without intrauterine devicesand with the normal menstrual cycle were selected as control group. Patients in the abnormal uterine bleeding group and non-bleeding group underwenttheremoval of intrauterine device and scraping endometrial tissues within 4-7 days after menstruation; patients in the control group underwentscraping endometrial tissues within 4-7 days after menstruation. The copper ion content in endometrial tissues was measured by atomic absorption method;expression of endometrial vascular endothelial growth factor detected by immunohistochemical stainingand the microvessel density counted.Additionaly, the corrosionratio of copper ion in the simulated uterine fluid was detected using flame atomic absorption spectroscopy method.
<br> RESULTS AND CONCLUSION:The content and corrosionratio of copper ion in theuterine abnormal bleeding group was significantly higher than thoseinthe non-bleeding group (P< 0.05), and the copper ion content of no bleeding group was significantly higher than that of control group (P< 0.05). The amount of copper in the intrauterine device of abnormal uterine bleeding group was higher than thatin non-bleeding group (P< 0.05).Compared with the control andnon-bleeding groups, the vascular endothelial growth factor expression and microvessel density were significantly increasedin the uterine abnormal bleeding group(P< 0.05); and the control group and non-bleeding group had no significant differencesinthe vascular endothelial growth factor expression and microvessel density. Furthermore, there was a positive correlationofthevascular endothelial growth factor expressionwithmicrovessel density and copper ion content. In conclusion, after implantation ofcopper/low-density polyethylene nanocompositesintrauterine device, the higher copper ion concentration in endometrial tissues may lead to the over-expression of vascular endothelial growth factorthatincreases the endometrial microvessel density througha variety of ways, and promotemicrovesselexpansion and congestion, finaly resulting in abnormal uterine bleeding.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 5057-5063 )