Tissue-engineered acellular matrix material：preparation and application in articular cartilage repair
Zhao Yu-guo
Li Ming-ming
Abstract：BACKGROUND:It has beenreportedthat tissue-engineered acelular matrix can induce and promote epithelial cels and smooth muscle celstoevolveinto a part of bodyin vivocompared with the normal extracelular matrix.
<br> OBJECTIVE:To investigate the effect of tissue-engineered acelular matrix in articular cartilage repair.
<br> METHODS:Totaly 30 New Zealand rabbits were randomly alottedto fibroid tissue andacelular matrix groups (n=15 per group), and then articular cartilage defect models,4mmin diameter,were established at the white rabbitfemoral condyle. Acelular cartilage matrix scaffold was prepared using bovine knee cartilage, and model rats in the acelular matrix group were repaired with acelular cartilage matrix scaffold and the others in the fibroid tissue group repaired with fibroid tissues. Finaly, repair effects between two groups were compared.
<br> RESULTS AND CONCLUSION:The dark blue and porous tissue-engineered acelular matrix material could be found, with a diameter of 5mm and moderate hardness, and exhibited certain flexibility after cross-linking. Hematoxylin-eosin staining showed that cel debris,blue-stainednuclear materials and residual extracelular matrix disappeared. Toluidine blue staining found that the porosity of the blue scaffold was 90%, and the sweling ratio was (1314±337)%. The absorbance value in the acelular matrix group was significantly higher than that in the fibroid tissue group at 1, 3, 5, 7 and 9 days (P< 0.05). In the fibroid tissue group,defectsfiled withnewborn fibrous scars were overt. By contrast, in the acelularmatrix group, the white tissuescovered the defect regionwith smooth surface,and the woundwas basicaly healed,withanunclearboundaryafter 12weeks. Moreover, blue-stained, smal flattened cels appeared, subchondral bone structurewasconnected wel with the cartilage, and the scaffold was directly degraded. In conclusion, the tissue-engineered acelular matrix material exhibits good biocompatibility, cel adsorption and hydrophilicity, and can promote the defect repairafterarticular cartilage injury.Therefore, it is a suitable substitute for articular cartilage repair.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5051-5056 )