Biological properties of nanohydroxyapatite composite for dental restoration
Huang Tao
Chen Han
Abstract：BACKGROUND:Nanohydroxyapatite composition issimilarto that of the natural bone, and because of its characteristics of nanomaterials, it has been widely used in the bone defect repair.
<br> OBJECTIVE:To observe the effect of nanohydroxyapatite composite materials in the repair of alveolar bone defect in animal models by detectingits cytotoxicity.
<br> METHODS:(1)In vitroexperiment: nanohydroxyapatite extracts with concentrations of 10%, 50%, 100% (or hydroxyapatite leaching extracts) were used to culture periodontal ligament fibroblast-like cels and L-929 cels for 7 days, respectively, folowed by cel cytotoxicity analysis. (2)In vivoexperiment: 45 adult rabbits were used to make animal models of alveolar bone defects and then were randomized into three groups: experimental group implanted with nanohydroxyapatite, control groupwith hydroxyapatite, and blank control group with no implant material. New bone formation was observed at 1, 2 and 3 weeks after operation.
<br> RESULTS AND CONCLUSION:(1)In vitroexperiment: after directly cultured with 10%, 50%, 100%nanohydroxyapatite extracts, the cel cytotoxicity was graded 0 to 1, indicating no presence of cytotoxicity. (2)In vivoexperiment: in the three groups, the percentage of new bone area accounting for the total visual field was gradualy in a rise over time. Moreover, the amount of new bone tissues was higher in the experimental group than the other two groups at different time (P< 0.05). These findings demonstrate that the nanohydroxyapatite materials have no cytotoxicity that can be used to promote the repair of alveolar bone defects.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5045-5050 )