Biocompatibility of rabbit annulus fibrosus-derived stem cells with genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels

Liu Chen; Zhao Quan-lai; Wang Ling-ting; Wang Hong; Liu Ping; LiBin; Xu Hong-guang

doi:10.3969/j.issn.2095-4344.2016.21.015

Abstract：BACKGROUND:To improve the mechanical properties and uncontrolability of degradation of decelularized matrix, wemanufactured genipin cross-linked decelularized annulus fibrosus matrix/chitosan hydrogels as annulus fibrosus tissue-engineereds caffold. <br> OBJECTIVE:To observe the biocompati bility of annulus fibrosus-derived stem cels with genipin cross-linked decelularized annulus fibrosus matrix/chitosan hydrogels, and the degradation of hydrogels in vivo. <br> METHODS:Genipin cross-linked decelularized annulus fibrosus matrix/chitosan hydrogels caffoldswere prepared, and the internal structure and hydrophilic property of hydrogels was detected by scanning electron microscopy and contact angle meter, respectively. Afterwards, passage 1 rabbit annulus fibrosus-derived stem cels were seeded on thescaffoldsfor 3 days, cel morphology was observed by inverted immun of luorescence microscopy and scanning electron microscopy after cytoskeleton staining, andadditionaly, curve of cel growth was obtained. In the meanwhile, thescafflodswere transplanted into theintermuscular space of New Zealand white rabbits, and the degradation and inflammatory reaction of the hydrogels were observedafter4 weeks. <br> RESULTS AND CONCLUSION:The prepared hydrogelsc affoldshowed porous and network structure and the contact angle was (39.94±1.61)°; annulus fibrosus-derived stem cels adhered wel on thescaffold surfacewithafaster growth rate. Furthermore, at 4 weeks after trans plantation, the hydrogelin vivowas degraded, and inflammatory cels appeared. These findings indicate that genipin cross-linked decelularized annulus fibrosus matrix/chitosan hydrogels have good biocompati bility.