Distribution and differentiation of embryonic liver stem cells in mice after intrahepatic transplantation

Jiang Si-feng

doi:10.3969/j.issn.2095-4344.2016.19.017

Abstract：BACKGROUND:As many factors can lead to liver injury, we attempt to use the“therapeutic liver regeneration”technology in clinical treatment of liver diseases by promoting liver regeneration. OBJECTIVE:To investigate distribution and differentiation of embryonic liver stem cel s in mice after intrahepatic transplantation via a transplantation approach. METHODS:Liver injury models were prepared in 20 BALB/c mice, and then randomly equivalently assigned into two groups:70%partial hepatectomy with intrahepatic transplantation with 1×105 embryonic liver stem cel s in control group;therapeutic liver regeneration model plus intrahepatic transplantation with 1x105 embryonic liver stem cel s in observation group. At 1 and 2 weeks after cel transplantation, the liver parenchyma of mice was observed. And at 2 weeks, both of the two groups underwent confocal immunofluorescence assay. Besides, blood samples of mouse tail vein were col ected to detect levels of serum albumin. RESULTS AND CONCLUSION:At 1 week after cel transplantation, in the liver parenchyma, green fluorescence was sparsely distributed in the two groups, and the distribution density had no significant difference between the two groups;at 2 weeks after cel transplantation, hepatic cord-like structures appeared in the liver parenchyma of two groups, and the green fluorescence distribution in the control group was limited, but significantly expanded in the observation group. At 2 weeks after cel transplantation, positive albumin expression in the liver parenchyma was significantly higher in the observation group than in the control group, and there was no significant difference in levels of serum albumin between two groups (P>0.05). To conclude, after transplantation of embryonic liver stem cel s in the therapeutic liver regeneration model mice hepatocytes can be effectively integrated into the host hepatic plate, differentiate in the liver, and partial y trigger the function of hepatocytes.