Chondrocyte-like differentiation of synovial mesenchymal stem cells co-cultured with chondrocytes on a three-dimensional scaffold
Abstract：BACKGROUND:The co-culture of chondrocytes and synovial mesenchymal stem cels can induce the cartilage differentiation of synovial mesenchymal stem celsin vitro, but the cel differentiation induced by co-culturein vivo is rarely reported. <br> OBJECTIVE:To investigate the chondrogenic differentiation of synovial mesenchymal stem cels co-cultured with chondrocytes on the chitosan/type I colagen composite scaffolds after being transplanted into the subcutaneous layer of Sprague-Dawley rats. <br> METHODS: The synovial mesenchymal stem cels and chondrocytes harvested from the synovial membrane and articular cartilage of Sprague-Dawley rats were obtained by enzyme digestion method and cultured respectively. Passage 3 synovial mesenchymal stem cels and passage 2 chondrocytes, which were divided into four groups: group A (chondrocytes alone), group B (synovial mesenchymal stem cels alone), group C (ratio of synovial mesenchymal stem cels:chondrocytes=1:2) and group D (scaffold material without cels), were cultured on chitosan/type I colagen composite scaffolds and transplanted into the subcutaneous layer of rats folowed by morphological observation and immunohistochemical staining at 4 and 8 weeks. . <br> RESULTS AND CONCLUSION:After 4 and 8 weeks, the discoid-like scaffold was visible. The immunohistochemical staining of type II colagen and the toluidine blue staining of aggrecan were significantly positive in groups A and C. These results show that the co-culture of synovial mesenchymal stem cels and chondrocytes on the scaffold in vivocan form cartilage-like tissues.
Keywords：Synovial MembraneMesenchymal Stem CelsChondrocytesCoculture TechniquesColagen Type IITissue Engineering
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1544-1550 )