Folic acid magnetic nanoparticles containing cisplatin inhibit the growth of human laryngeal cancer Hep-2 cells in a non-cytotoxic manner

Zhang Hai-zhong; Huang Ai-ping

doi:10.3969/j.issn.2095-4344.2016.08.007

Abstract：BACKGROUND: Chemotherapy drugs can kil both cancer cel s and normal tissue cel s. Molecular targeted therapy can reduce or avoid toxicity of chemotherapy drugs to normal cel s. OBJECTIVE: To study the therapeutic effect of folic acid magnetic nanoparticles containing cisplatin on laryngeal cancer. METHODS: Folic acid-cis-diaminodichloroplatinum (II)-aldehyde sodium alginate-magnetic nanoparticles (FA-CDDP-ASA-MNPs) were prepared. The FA-CDDP-ASA-MNPs, CDDP and FA-ASA-MNPs were cultured together with human laryngeal cancer Hep-2 cel s. To detect the effect of FA-CDDP-ASA-MNPs, CDDP and FA-ASA-MNPs on human laryngeal cancer Hep-2 cel s, MTT, flow cytometry and transmission electron microscopy examination were used in vitro. RESULTS AND CONCLUSION: Both FA-CDDP-ASA-MNPs and CDDP could inhibit the growth of human laryngeal cancer Hep-2 cel s, and there was no difference in their inhibitory rates (P > 0.05). FA-CDDP-ASA-MNPs and CDDP showed increased inhibitory rates of human laryngeal cancer Hep-2 cel s with the increase of CDPP concentration. The half maximal inhibitory concentrations of CDDP for inhibiting Hep-2 cel s were significantly different at 24 and 48 hours after culture (P < 0.05). Both FA-CDDP-ASA-MNPs and CDDP could cause apoptosis in Hep-2 cel s, and the number of apoptotic cel s was increased with the increase of drug concentrations (P < 0.05), but there was no difference between two kinds of drugs (P > 0.05). FA-CDDP-ASA-MNPs and CDDP at low concentrations could increase the percentage of cel s at G0/G1, but decrease the cel proportion at S and G2/M phases (P < 0.05). Under transmission electron microscope, Hep-2 cel s with the intake of FA-CDDP-ASA-MNPs appeared to have notable apoptosis. These findings suggest that folic acid magnetic nanoparticles containing cisplatin can inhibit the growth of human laryngeal cancer Hep 2 cel s, with no influence on the efficacy of cisplatin and with no cytotoxic effect.