Transplantation of human amnion-derived mesenchymal stem cells alleviates ischemia-reperfusion-induced acute lung injury after cardiopulmonary bypass

doi:10.3969/j.issn.2095-4344.2016.01.013

Abstract：BACKGROUND:In recent years, mesenchymal stem cels exhibit a good prospect in organ or tissue repair and therefore, and therefore, cel transplantation based on mesenchymal stem cel plasticity can promote cel regeneration and functional recovery from lung injury after cardiopulmonary bypass. OBJECTIVE:To investigate the effects of human amnion-derived mesenchymal stem cels (hAMSCs) transplantation on ischemia-reperfusion-induced acute lung injury in dogs after cardiopulmonary bypassand its mechanism for regulating inflammatory cytokines. METHODS:Eighteen adult healthy mongrel dogs were randomly divided into three groups (n=6 per group): black group (cardiopulmonary bypass with 1 mL physiological saline injectionvia the femoral vein without blocking the aorta), control group (cardiopulmonary bypass with blocking the aorta for 1 hour and then opening the aorta for 15 minutes plus 1 mL physiological saline injectionvia the femoral vein), experiment group (cardiopulmonary bypass with blocking the aorta for 1 hour and then opening the aorta for 15 minutes plus femoral vein injection of 1 mL physiological saline containing 2×107 hAMSCs). Arterial blood samples of 2 mL were taken to calculate oxygenation index and respiratory index before cardiopulmonary bypass (T1), 15 minutes (T2), 1 hour (T3), 2 hours (T4), 3 hours (T5) after opening the aorta. 8 mL intravenous blood samples were taken to detect the serum tumor necrosis factor α, matrix metaloproteinase-9, interleukin-8 and interleukin-10 by ELISA. Meanwhile, western blot assay was used to detect the expression of nuclear factor-κB in lung tissues, and histopathological changes of lung tissues observed under optical microscope. RESULTS AND CONCLUSION:Compared with the control group, the oxygenation index was significantly increased in the experimental group at 2 and 3 hours after transplantation, and the respiratory index was remarkably decreased at 1, 2, 3 hours after transplantation. Compared with the control group, the levels of tumor necrosis factor α, matrix metaloproteinase-9 and interleukin-8 were significantly decreased in the experimental group, but the level of interleukin-10 was increased in the experimental group. Compared with the blank group, the expression of nuclear factor-κB was significantly strengthened in the control group at 3 hours after opening the aorta, but it was decreased significantly after intravenous injection of hAMSCs. After opening the aorta, the pathological changes in the experimental group were remarkably milder than those in the control group, and a smal amount of red blood cels and neutrophils exuded, with telangiectasia, and congestion, were found in the experimental group. These findings indicate that hAMSCs transplantation can mitigate ischemia-reperfusion- induced acute lung injury after cardiopulmonary bypass by adjusting the level of nuclear factor-κB in lung tissues and release of tumor necrosis factor α, matrix metaloproteinase-9, interleukin-8 and interleukin-10. Cite this article: Qiang Y, Liang GY, Yu LM, Qi B, Gao ZY.Transplantation of human amnion-derived mesenchymal stem cels aleviates ischemia-reperfusion-induced acute lung injury after cardiopulmonary bypass. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):70-77.