Therapeutic effect of Fukeyangkun pills and bone marrow mesenchymal stem cells transplantation on thin endometrium

doi:10.3969/j.issn.2095-4344.2016.01.012

Abstract：BACKGROUND:Studies have found that endometrial cels from bone marrow donors can be detected in the endometrium of female patients after bone marrow suppression. OBJECTIVE: To investigate the effect ofFukeyangkun pils combined with bone marrow mesenchymal stem cels (BMSCs) transplantation in the treatment of thin endometrium. METHODS:Thirty female rats at rutting period were randomized into control, model,Fukeyangkun pils, cel transplantation and combined group (n=6 per group). Rat models of thin endometrium were made in the latter five groups. Rats in the six groups were respectively subjected to routine feeding, tail vein injection of normal saline, tail vein injection of bone marrow mesenchymal stem cels (1 mL) at 6 hours and 10 days after modeling, intragastric administration of 5 mL/kgFukeyangkun pil solution for continuous 20 days, or tail vein injection of bone marrow mesenchymal stem cels (1 mL) at 6 hours and 10 days after modeling plus intragastric administration of 10 mL/kgFukeyangkun pil solution for continuous 20 days. At 21 days after modeling, hematoxylin-eosin staining was used to observe the morphological changes of the endometrical tissues and measure the endometrium thickness. The expression of cytokeratin and vimentin was determined by western blot assay. RESULTS AND CONCLUSION:Compared with the model group, the endometrium thickness and the expression of cytokeratin and vimentin (P< 0.05) were increased successively in theFukeyangkun pils group, cel transplantation group, and combined group to different extents. Of these groups, the endometrium thickness and the expression of cytokeratin and vimentin in the combined group were the most close to normal levels. Our data demonstrate that bone marrow mesenchymal stem cel transplantation can induce regeneration of the endometrial cels and repair endometrial tissue. Furthermore, treatment ofFukeyangkun pils obviously augments the repair effect of bone marrow mesenchymal stem cel transplantation on thin endometrium. Cite this article:Han R, Dai N, Lin Y, Lin T.Therapeutic effect of Fukeyangkun pils and bone marrow mesenchymal stem cels transplantation on thin endometrium. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):65-69.