ABCE1 gene silencing effect on biological characteristics of human cervical cancer cells XB1702

Li Ping; Wang De-hua

doi:10.3969/j.issn.2095-4344.2015.37.013

Abstract：BACKGROUND:With the development of genetic engineering and tumor molecular biology, gene therapy has acted as a new treatment mode for tumors. OBJECTIVE:To investigate the impact of gene silencing ABCE1 on growth, proliferation and migration of human cervical cancer cel s XB1702. METHODS:siRNA sequences of ABCE1 were designed and synthesized to transfect XB1702 cel s using LipofectamineTM 2000. Cel s transfected with NC siRNA carrier served as controls, and untransfected cel s acted as blank group. After RNA interference, the expression of ABCE1 mRNA and protein was detected by western blod assay;cel cycle was detected using flow cytometry;cel counting kit-8 test, scratch healing assay and cel invasion assay were used to evaluate the effects of ABCE1 silence gene on XB1702 cel proliferation, migration and invasion ability. RESULTS AND CONCLUSION:ABCE1 mRNA and protein expression in the experimental group was significantly lower than that in the control group and blank group (P<0.05). Growth rate in the experimental group was significantly slowed, cel s were arrested in G 0/G 1 phase, and the number of S-phase cel s was reduced. Compared with the control group and blank group, XB1702 cel proliferation was inhibited significantly in the experimental group, and cel migration and invasion was significantly decreased (P<0.05). These results showed that specific interference with gene expression of ABCE1 can inhibit the migration of human cervical cancer cel s XB1702, and restrain tumor cel proliferation;therefore, the siRNA sequence of ABCE1 may be an effective target for treatment of cervical cancer.