Proliferation and differentiation of transplanted neural stem cells in the damaged striatum of Parkinson’s disease rats

Liu Zhen-hua; Wang Shi-jun

doi:10.3969/j.issn.2095-4344.2015.36.019

Abstract：BACKGROUND:In recent years, cel transplantation therapy is widely used in the treatment of Parkinson’s disease with satisfactory outcomes. OBJECTIVE:To investigate the proliferation and differentiation of transplanted neural stem cel s in Parkinson’s disease rats, thereby providing a new theoretical basis for the treatment of Parkinson’s disease. METHODS:Neural stem cel s from the brain tissues of rats were isolated and cultured in vitro using col agenase digestion method. Unilateral rat model of Parkinson’s disease was made by 6-hydroxydopamine method, and the successful rat models were divided into model group and cel transplantation group, with seven rats in each group. The rats in the model group were given 4 mL normal saline at the damaged striatum, and those in the cel transplantation group were injected 5μL green fluorescent protein-labeled neural stem cel s (1×109/L) into the damaged striatum. RESULTS AND CONCLUSION:After cel transplantation, the positive area of tyrosine hydroxylase was significantly lower in the cel transplantation group than the model group. Compared with the model group, the expression level of Ptx3 mRNA was significantly higher in the cel transplantation group at 7, 14, 28 days after cel transplantation and the expression of SHH mRNA was significantly higher in the cel transplantation group at 14 and 28 days after cel transplantation. However, there was no difference in the expression of Nurrl mRNA between the two groups. These findings indicate that neural stem cel s injected into the damaged striatum of rats can differentiate into dopaminergic neurons, which bring a breakthrough in the treatment of Parkinson’s disease.