Ganglioside combined with bone marrow mesenchymal stem cells transplantation for treatment of traumatic brain injury

Huang Yong-hui

doi:10.3969/j.issn.2095-4344.2015.36.013

Abstract：BACKGROUND:Bone marrow mesenchymal stem cel s can secrete neurotrophic factors in vitro, and can also be differentiated into neurons, thereby contributing to the repair of traumatic brain injury. However, the short life cycle of bone marrow mesenchymal stem cel s influences their protective effects on the damaged brain tissues. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation combined with ganglioside in rats with traumatic brain injury. METHODS:Sixty Wistar rats were used to make severe traumatic brain injury models using a hydraulic head injury instrument, and then randomized into three groups:1 mL DMEM, 1 mL bone marrow mesenchymal stem cel suspension (1×1010/L), 1 mL bone marrow mesenchymal stem cel suspension (1×1010/L) combined with ganglioside solution (30 mg/kg) were injected respectively in model group, transplantation group and combined group, once a day, total y for 3 days. Neurological behavior scores were observed according to Longa method at 24 hours after modeling and at 3 days, 1, 2, 3, 4 weeks after cel transplantation. At 3 days after cel transplantation, RT-PCR and western blot assay were employed to detect aquaporin 4 mRNA and protein expressions. At 1 week after transplantation, hematoxylin-eosin staining was performed for pathological observation of the damaged brain tissues. RESULTS AND CONCLUSION:At 3 days, 1, 2, 3, 4 weeks after cel transplantation, the neurological behavior scores were ranked as fol ows:combined group<transplantation group<model group (P<0.05). At 3 days after cel transplantation, the mRNA and protein expression of aquaporin 4 was ranked as fol ows:model group>transplantation group>combined group (P<0.05). Hematoxylin-eosin staining showed that the recovery of brain tissue was better in the combined group than the model and transplantation groups (P<0.05). These findings indicate that bone marrow mesenchymal stem cel s combined with ganglioside can significantly improve the neurological behavior function of rats with traumatic brain injury.