Allogeneic adipose-derived stem cells combined with nano collagen-based bone for repair of ulna bone defects

Shen Ling; Wang Xi-you; Chen Ping; Yu Tong

doi:10.3969/j.issn.2095-4344.2015.32.014

Abstract：BACKGROUND:With the continuous development of medical model, high-level treatment and rehabilitation for bone defects are required. Therefore, bone tissue engineering technique based on stem cel culture has become a more important component of bone defect repair. <br> OBJECTIVE:To explore and analyze the feasibility of alogeneic adipose-derived stem cels combined with nano colagen-based bone for repair of ulna bone defects. <br> METHODS:Seventy adult female New Zealand rabbits were selected, 10 of which were used to isolate and culture adipose-derived stem cels, another 60 were randomized into scaffold group, scaffold+bone autograft group, scaffold+alogeneic adipose-derived stem cels group, with 20 rabbits in each group. The middle part of the ulna was resected to construct 12-mm bone defect models in the three groups, and then, corresponding materials were implanted. At 6, 9 and 12 weeks after implantation, bone mineral density was detected; at 14 weeks after implantation, the rates of solid fusion rate, non-solid fusion, and false joint were observed. <br> RESULTS AND CONCLUSION:Compared with the other two groups, the rate of solid fusion was significantly higher and the rates of non-solid fusion and false joint were lower in the scaffold+alogeneic adipose-derived stem cels group (P < 0.05). Compared with the scaffold group, the rate of solid fusion was higher and the rates of non-solid fusion and false joint were significantly lower in the scaffold+bone autograft group (P< 0.05). The bone mineral density increased dramaticaly at 6, 9, 12 weeks after implantation of scaffold+alogeneic adipose-derived stem cels, which was significantly higher than that in the scaffold group and scaffold+bone autograft group (P < 0.05). Compared with the scaffold group, the bone mineral density in the scaffold+bone autograft group was significantly higher (P < 0.05). These findings indicate that alogeneic adipose-derived stem cels combined with nano colagen-based bone has exact fusion effects with a higher bone mineral density.