Dendritic cell costimulatory molecule expression and immunosuppressant intervention

Yang Xiao-yong; Yao Qing-chun; Wang Wei; Qi Man; Hong Xi-ying; Liu Chen-mei; Yin Hang; Liu Hang; Ren Liang; Hu Xiao-peng; Zhang Xiao-dong

doi:10.3969/j.issn.2095-4344.2015.24.020

Abstract：BACKGROUND:Previous studies on immunosuppression and anti-rejection after organ transplantation mainly focused on effects of T lymphocytes-mediated immune response and immunosuppressive agents on T lymphocytes. Effects of dendritic cel s were unclear. The manifestation and mechanism of immunosuppressive agent effects on dendritic cel s are not identical. OBJECTIVE:To compare the effects of different immunosuppressive agents on expression and function of costimulatory molecules of dendritic cel s, and to explore the mechanism of action of immunosuppressive agents. METHODS:20μg/L rapamycin, 0.04 mg/L mycophenolate, 10μg/L tacrolimus and 1 mg/L cyclosporine A were separately added during bone marrow cel s of C57BL/6 mice were differentiated into dendritic cel s. RESULTS AND CONCLUSION:Flow cytometry results revealed that CD40 expression in each group:rapamycin<tacrolimus<cyclosporine A<mycophenolate mofetil (P<0.01);CD86 expression:rapamycin<tacrolimus<cyclosporine A=mycophenolate mofetil (P<0.01). There was no significant difference in CD80, CD11c and major histocompatibility complex II expression among groups (P>0.05). One-way mixed lymphocyte reaction results displayed dendritic cel costimulatory T cel proliferation in each group:rapamycin<mycophenolate mofetil<tacrolimus=cyclosporine A (P<0.05). The results confirmed that rapamycin, tacrolimus, cyclosporine A, and mycophenolate mofetil exerted immunosuppression by suppressing CD40 and CD86, especial y rapamycin. Above medicines do not noticeably inhibit major histocompatibility complex II expression, but suppress T cel proliferation, and the inhibitory effect of rapamycin on cel proliferation is strongest.