Activated expression of matrix metalloproteinase is involved in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide
Yan Guang-hua
Qin Hua
Abstract：BACKGROUND:Matrix metal oproteinases and their inhibitors are proteolytic enzymes contaning Zn+, and involved in extracel ular matrix degradation and tissue remodeling of a variety of tissues. OBJECTIVE:To observe the effects of matrix metal oproteinases in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide. METHODS:MC3T3-E1 cel line was divided into four groups randomly:control group, low-dose lipopolysaccharide group (1μmol/L), moderate-dose lipopolysaccharide group (10μmol/L), and high-dose lipopolysaccharide group (100μmol/L). The proliferation rate in each group was analyzed. Matrix metal oproteinase 2, 3, 9 and matrix metal oproteinase inhibitor 1 and 2 expressions were detected. RESULTS AND CONCLUSION:The proliferation rate was increased greatly after medication of lipopolysaccharide in time-dependent and concentration-dependent manners. Moreover, the expressions of matrix metal oproteinases and their inhibitors were apparently enhanced, and showed significant differences. Results indicate that the enhanced expressions of matrix metal oproteinases participated in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide.
Keywords：LipopolysaccharidesMatrix Metal oproteinasesOsteoblasts
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3865-3869 )