Effect of Dll4 at different concentrations on the biological functions of extravillous trophoblasts
Luo Qing-qing
Abstract：BACKGROUND:Extravil ous trophoblasts exert a crucial effect on early pregnancy. When extravil ous trophoblasts function abnormal y, many pregnancy diseases can develop, such as preeclampsia, fetal growth restriction and placental implantation. OBJECTIVE:To investigate the effect of Dl 4, a ligand of Notch signaling family, on biological functions of extravil ous trophoblasts. METHODS:Human extravil ous trophoblast cel lines, HTR-8/SVneo, were cultured with human recombined protein Dl 4 of different concentrations (0, 0.125, 0.25, 0.5, 1.0 mg/L). Cel viability was tested by cel counting kit-8 assay. Transwel assay was applied to examine the changes in cel migration and invasion ability. RESULTS AND CONCLUSION:Dl 4 could significantly improve migration and invasion abilities of extravil ous trophoblasts, but had no effect on cel viability. In addition, Dl 4 regulated the invasion ability of extravil ous trophoblast in a dose-dependent manner. Above al , Dl 4 can play an important role in normal pregnancy by regulating the biological functions of extravil ous trophoblasts.
Keywords：ReceptorsNotchChorionCel Movement
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3860-3864 )