Tissue microarray detection for astrocyte elevated gene-1 and its correlation with pathological factors in primary hepatocellular carcinoma

doi:10.3969/j.issn.2095-4344.2015.20.006

Abstract：BACKGROUND:Astrocytes elevated gene-1 is an important molecular marker for early tumor metastasis, which is likely to become the molecular target in the clinical treatment of hepatocelular carcinoma. OBJECTIVE:To investigate the astrocyte elevated gene-1 expression level in the tumor tissues of patients with hepatocelular carcinoma, and to predict its role in the minimaly invasive therapy. METHODS:Tissue microarray and immunohistochemistry methods were applied to detect astrocyte elevated gene-1 protein expression level in the tumor tissues of patients with hepatocelular carcinoma, and their clinical-pathological data were colected for correlation analysis with the astrocyte elevated gene-1 protein expression level. RESULTS AND CONCLUSION:The protein expression level of astrocyte elevated gene-1 was closely related to clinical stage and tissue differentiation degree in patients with hepatocelular carcinoma. In addition, the high expression of astrocyte elevated gene-1 protein was correlated with the positive rate of Ki-67 and tumor micro-vessel density. However, the protein expression had no correlation with gender and age in these patients. Taken together, the protein expression level of astrocyte elevated gene-1 is correlated with conventional malignant indexes, which is of great significance for the judgment of hepatocelular carcinoma.Subject headings: Carcinoma, Hepatocelular; Microchip Analytical Procedures; Ki-67 Antigen; Carcinoma Funding: the National Natural Science Foundation of China, No. 81472328; YouAN Fund for Liver Diseases and AIDS, No. BJYAH-2011-034; the National Science and Technology Support Program, No. 2012BAI15B08; the National Special Fund for Infectious Diseases, No. 2012ZX10002015-002