Neural stem cells:in vitro culture, identification and differentiated phenotype

doi:10.3969/j.issn.2095-4344.2015.19.018

Abstract：BACKGROUND:Neural stem cels can repair the damaged central nervous system structure and function, which have broad application prospects. It can be realized by studies on in vitro culture, identification and differentiation phenotype of neural stem cels. OBJECTIVE: To observe the biological characteristics and differentiated phenotypes of neural stem cels under induction in vitro. METHODS:Neural stem cels were extracted from the hippocampus and olfactory bulb of newborn mice. After three generations, neural stem cels were labeled with 5-bromo-2-deoxyuridine (BrdU) and identified by immunofluorescence staining of BrdU, nestin and Hochest33258. The differentiation of neural stem cels was inducedin vitro and identified by immunofluorescent staining of BrdU,β-tubulinⅢ, glial fibrilary acidic protein and Hochest33258. RESULTS AND CONCLUSION:After passage, neural stem cels from the hippocampus and olfactory bulb of newborn mice could be aggregated into neurospheres that were positive for nestin and BrdU. Under induced differentiationin vitro, neural stem cels gradualy turned into daughter cels which positively expressedβ-tubulin III or glial fibrilary acidic protein. These findings suggest that neural stem cels have strong self-renewal capacity and have the tendency to form neurospheres during culturein vitro; underin vitro induction, they can differentiate into neurons and astrocytes through asymmetric cel proliferation and differentiation.