Effect of adipose stem cell transplantation on the neuromuscular regeneration unit of Duchenne muscular dystrophy mice
Abstract：BACKGROUND:Currently, there is no effective treatment for Duchenne muscular dystrophy. Previous studies have shown that gene therapy and stem cel transplantation therapy are possible to be "cure" approaches. Therefore, this study intended to evaluate their efficacy in animal models, and to verify the hypothesis of neuromuscular regeneration unit. OBJECTIVE: To explore the efficacy and feasibility of adipose stem cel transplantation for Duchenne muscular dystrophy and to observe the effects of cel transplantation on muscle fibers, new vessels and nerve endings. METHODS:Adipose stem cels from mdx mice were isolated and cultured in vitro, and then modified by recombinant baculovirus folowed by implantation into mouse models of Duchenne muscular dystrophy. Serum creatine kinase level, muscle pathological changes and muscle dystrophin expression were detected in experimental animals after transplantation; immunofluorescence staining was used to detect vascular, muscle and nerve regeneration after cel transplantation. RESULTS AND CONCLUSION: After transplantation, dystrophin expression was found that could reduce and even reverse the pathological damage of muscles to a certain extent, thereby to reduce the serum level of creatine kinase. In addition, stem cels-derived muscle fiber, vasular endothelial cels and nerve ending were formed after cel transplantation. These evidences domenstrate that adipose stem cel transplantation is one of promising treatments for Duchenne muscular dystrophy.
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Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 3042-3048 )