Effects of different growth factors on biological behaviors of adipose tissue-derived stem cells

doi:10.3969/j.issn.2095-4344.2015.19.010

Abstract：BACKGROUND:Bone deficiency restricts the extensive use of oral implant restoration. How to promote stem cel migration, adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research. OBJECTIVE:To observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose tissue-derived stem cels culturedin vitro and to screen the optimal combined factors. METHODS: The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cels were cultured by enzyme digestion method. Passage 3 cels were randomly divided into five groups: transforming growth factor β1+platelet-derived growth factor AB group (group 1), platelet-derived growth factor AB+vascular endothelial growth factor group (group 2), transforming growth factor β1+vascular endothelial growth factor group (group 3), transforming growth factor β1+platelet-derived growth factor AB+vascular endothelial growth factor group (group 4), and blank control group.In vitro cel migration ability was examined by Transwel chamber. Cel proliferation and adhesion abilities were examined by cel counting kit-8 and cel adhesion assay, respectively. RESULTS AND CONCLUSION:Results from the Transwel test showed that the migration number of rabbit adipose tissue-derived stem cels showed significant differences among the former four groups (P < 0.05), which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cels. In the cel adhesion assay, there were significant differences in the number of adherent cels among the former four groups (P < 0.05), and the group 3 showed the most adherent cels that dramaticaly improved the adhesion of adipose tissue-derived stem cels. Compared with the blank control group, the absorbance values in the groups 1-4 were significantly higher at days 1, 3, 5, 7 (P < 0.05), and the group 2 showed the highest absorbance value, indicating the combination of platelet-derived growth factor AB and vascular endothelial growth factor significantly promoted the proliferation of rabbit adipose tissue-derived stem cels.