Migration, homing and differentiation of endogenous bone marrow mesenchymal stem cells after myocardial infarction

doi:10.3969/j.issn.2095-4344.2015.19.005

Abstract：BACKGROUND:Most of studies have focused on the transplantation and regeneration of exogenous stem cels-derived myocardial cels after myocardial infarction, but relatively few studies address the migration, homing and differentiation of endogenous stem cels. OBJECTIVE:To observe the migration, homing and differentiation of endogenous bone marrow mesenchymal stem cels after myocardial infarction. METHODS:Adult female C57BL/6 mice were randomized into two groups: myocardial infarction group (n=4), in which, the left anterior descending branch of the coronary artery was ligated to establish acute myocardial infarction models at 4 weeks after bone marrow remodeling, and 1 week later, the model mice were sacrificed; control group (n=3), in which, the mice were subject to bone marrow remodeling and then sacrificed after 4 weeks. Heart tissues were extracted from two groups folowed by immunofluorescent detection of Troponin I expression, and the distribution and differentiation of bone marrow mesenchymal stem cels positive for green fluorescent protein after myocardial infarction were observed. RESULTS AND CONCLUSION:After histological and immunohistochemical examination, bone marrow mesenchymal stem cels positive for green fluorescent protein were observed in both myocardial infarction group and control group, while more positive cels were seen in the myocardial infarction group. Some bone marrow mesenchymal stem cels in both two groups were positive for green fluorescent protein, Troponin I and PI. However, more triple-positive cels were observed in the myocardial infarction group compared to the control group. Endogenous bone marrow mesenchymal stem cels can migrate, home to the damage myocardium and acquire cardiogenic phenotype after myocardial infarction.