Osteogenic differentiation of pluripotent stem cells induced by akermanite extracts

Du Qing-hua; Cao Jun-kai; Dong Xi-xi; E Ling-ling; Wei Li-jun

doi:10.3969/j.issn.2095-4344.2015.14.018

Abstract：BACKGROUND:Akermanite belongs to silicate bioactive ceramics, with degradation in vivo, and its ionic products can induce osteogenic differentiation, which is a good choice for bone tissue engineering scaffold. OBJECTIVE:By studying the different concentrations of akermanite extracts on the proliferation and osteogenic differentiation of pluripotent stem cel s, to determine the optimum concentration of akermanite extract to inducing the osteogenic differentiation of pluripotent stem cel s. METHODS:MTT assay was used to detect the proliferation of pluripotent stem cel s cultured in different concentrations of akermanite extracts (1/2, 1/4, 1/8, 1/16, 1/32). After cultured with the extracts for 7, 14, 21 days, the culture supernatants were col ected to detect the levels of alkaline phosphatase, osteocalcin, type I col agen. RESULTS AND CONCLUSION:The MTT assay showed that the proliferation of pluripotent stem cel s was increased in a concentration-dependent manner after induction with akermanite extracts. The pluripotent stem cel s proliferated obviously at 3 days after induction, and then weaken at 7 days, but there was no difference at 3 and 5 days after induction. At 7 days after induction, the 1/4 extract had the best effect on promoting osteogenic differentiation of pluripotent stem cel s;the levels of alkaline phosphatase and osteocalcin were increased with time, especial y after induction with the 1/4 extract;but there was no expression of type I col agen. At 14 and 21 days after induction, the levels of alkaline phosphatase, osteocalcin, type I col agen were highest in the 1/4 extract group. These findings indicate that the 1/4 akermanite extract (Ca 2.37 mmol/L, Mg 1.12 mmol/L, Si 1.05 mmol/L) is the optimum to promote the osteogenic differentiation of pluripotent stem cel s.