Treatment of osteoarthritis using colony-forming cells in stromal vascular fraction of adipose tissue

Lv Pin-lei; Su Yue-han; Cao Yun; Wang Zheng

doi:10.3969/j.issn.2095-4344.2015.14.004

Abstract：BACKGROUND:Stromal vascular fraction digested from adipose tissue is a mixture of a variety of cel s, and clone-like cel components may have the real ability to maintain the stemness of mesenchymal stem cel s OBJECTIVE:To separate, passage and culture colony-forming cel s in the stromal vascular fraction of adipose tissue, and to test its ability to repair the knee cartilage in animal models. METHODS:Stromal vascular fraction isolated from fresh adipose tissue was cultured to col ect clone-like adherent cel s that were subject to digestion and passage until the third generation. A rabbit model of osteoarthritis was established surgical y, and the third generation of colony-forming cel s were transplantated into the articular cavity of model rabbits. Model rabbits with no cel transplantation served as controls. Cartilage regeneration in the knee joint of model rabbits were observed and compared. RESULTS AND CONCLUSION:At 3 days after transplantation of stromal vascular fraction cel s, clone-like cel colonies appeared in adherent cel s;at 5 days, the amount of clone-like cel colonies and surrounding single cel s were both increased. At the passage 3, the clone-like cel colonies were also visible, which were fusiform in shape. Gross observation and MRI examination showed that these colony-forming cel s could promote cartilage repair in the joint cavity, increase secretion of synovial fluid, and help to control inflammation and narrow ulceration area. Experimental findings are beneficial for optimizing the isolation and culture of adipose-derived mesenchymal stem cel s and provide a new way for the clinical treatment of osteoarthritis.