Effects ofXingnaojing on recombinant human tumor necrosis factor-mediated tissue-type plasminogen activator and plasminogen activator inhibitor-1 expression in human umbilical cord vein endothelial cells

Ouyang Hai-chun; Wu Wo-dong; Zhong Dong-mei; Wu Yan-xian; Li Wen-jie; Chen Xi-ming

doi:10.3969/j.issn.2095-4344.2015.11.015

Abstract：BACKGROUND:We have found thatXingnaojing can effectively inhibit mediators of inflammatory mediators and promote fibrinolytic activity in a rabbit model of myocardial ischemia and reperfusion. However, it is not completely clear what is the action of mechanism of fibrinolytic system activity in human umbilical vein endothelial cels. <br> OBJECTIVE:To investigate the effects ofXingnaojingon recombinant human tumor necrosis factor α-mediated tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in human umbilical cord vein endothelial cels. METHODS: The passages 3-5 of human umbilical cord vein endothelial cels were obtained, and were induced by adding 10 μg/L recombinant human tumor necrosis factor α in the culture medium.Xingnaojing in different concentrations (5, 10, 20 mL/L) were injected in theXingnaojing group, and fluvastatin (1 μmol/L) was added as positive control group. A blank control group of simple human umbilical cord vein endothelial cels culture was set up. After 24 hours of cultivation, the production of t-PA and PAI-1 in the cultured medium was determined by ELISA. The mRNA expression of t-PA and PAI-1 was measured by reverse transcript-PCR. RESULTS AND CONCLUSION:Compared with the blank control group, the secretion and mRNA expression of PAI-1 in the group treated with recombinant human tumor necrosis factor α(10μg/L) were significantly increased (P < 0.05); however, the secretion and mRNA expression of t-PA in the group treated with recombinant human tumor necrosis factorα (10μg/L) were significantly decreased (P < 0.05). The secretion and mRNA expression of t-PA were significantly higher in theXingnaojinggroup at different concentrations than in the recombinant human tumor necrosis factor α group (P < 0.05); however, the secretion and mRNA expression of PAI-1 were significantly lower in the former group than the latter group (P< 0.05), which was in a dose-dependent manner.Xingnaojing can effectively improve the fibrinolytic function of human umbilical cord vein endothelial cels mediated by recombinant human tumor necrosis factor α.