Platelet-derived growth factor-DD induces the proliferation and differentiation of rat renal fibroblasts into myofibroblasts

Zhao Jia; Sun Jian-ping; Gao Yan-xia; Tang Ni-na; Niu Meng; Cui Meng; Han Xiao-qing; Sui Ai-hua

doi:10.3969/j.issn.2095-4344.2015.11.010

Abstract：BACKGROUND:Platelet-derived growth factors can induce proliferation, migration, transformation and extracelular matrix expression of glomerular mesangial cels and renal interstitial cels. OBJECTIVE:To investigate the effect of activation of platelet-derived growth factor-DD/βR signaling pathway on the proliferation and α-smooth muscle actin expression in rat renal fibroblasts. METHODS: Normal rat kidney interstitial fibroblasts (NRK-49F) cultured in vitro were divided into the folowing groups by platelet-derived growth factor-DD concentrations: control, 1 μg/L, 10 μg/L, 50 μg/L, 100 μg/L. And NRK-49Fs were then divided into four groups according to the stimulation time of 50 μg/L platelet-derived growth factor-DD:control, 12 hours, 24 hours, 48 hours. The cel viability of the NRK-49Fs was assessed by Cel Counting Kit-8 after platelet-derived growth factor-DD administration. The mRNA and protein expression levels of <br> platelet-derived growth factor-βR and α-smooth muscle actin in al groups stimulated by different concentrations of platelet-derived growth factor-DD were determined by RT-PCR and western blot, respectively. RESULTS AND CONCLUSION:Platelet-derived growth factor-DD could facilitate the proliferation rates of the NRK-49Fs at a dose- and time-dependent manner as compared with the control groups. Platelet-derived growth factor-DD could stimulate the mRNA and protein expressions of platelet-derived growth factor-βR and α-smooth muscle actin in a dose-dependent manner. This suggest that the activation of platelet-derived growth facto-DD/βR signaling pathway can obviously promote the proliferation of NRK-49Fs as wel as tranformation into myofibroblasts.