c-fos/c-jun regulates extracellular matrix metalloproteinase 20 expression in ameloblasts
Tang Pei-juan
Wang Chang-lei
Gong Chun-mei
Tang Pei-qian
Hao Jian-zhong
Abstract：BACKGROUND:Matrix metaloproteinase 20 is a protease specificaly expressed in ameloblasts, which has an important role in dental enamel development. To study the regulatory mechanisms for matrix metaloproteinase 20 at the molecular level lays the foundation for further animal experiments. OBJECTIVE:To explore the regulatory effects of transcription factor c-fos/c-jun for matrix metaloproteinase 20 in mouse ameloblasts and to preliminarily confirm the role of c-fos/c-jun in enamel development. METHODS:First of al, a recombinant plasmid containing c-fos was established, and then dual-luciferase reporter assay system and RT-PCR were used to analyze the effects of c-fos, c-jun transfection of ameloblasts on the activity of matrix metaloproteinase 20. Furthermore, the effect of c-fos, c-jun on matrix metaloproteinase 20 was explored based on gene site-directed mutation and dual-luciferase reporter assay system. RESULTS AND CONCLUSION:Double luciferase report assay system and RT-PCR analysis showed that the mRNA expression of matrix metaloproteinase-20 was significantly upregulated after c-fos, c-jun transfection of ameloblasts, but c-fos/c-jun could not upregulate the transcriptional activity of matrix metaloproteinase 20 promoter when mutation occurred at AP1 binging site. These findings indicate that c-fos/c-jun has significant effects in regulating the mRNA expression of matrix metaloproteinase 20, which shows c-fos/c-jun plays an
<br> important biological meaning in enamel development.
Keywords：Proto-Oncogene Proteins c-fosMatrix Metaloproteinase 20Dental Enamel
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1673-1677 )